Chapter VI Part b: Production, purification and characterization of alkaliphilic mannanase by Exiguobacterium sp. VSG-1
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Optimization and purification of mannanase produced by an alkaliphilic-thermotolerant Bacillus cereus N1 isolated from Bani Salama Lake in Wadi El-Natron
An alkaliphilic-thermotolerant Bacillus cereus N1 isolated from Bani Salama Lake, Wadi El-Natron, Egypt, was proved to produce mannanase enzyme. Optimization of the fermentation medium components using Plackett-Burman design was applied. Glucose and inoculum size were found to be the most significant factors enhancing the production of the enzyme. On applying optimized medium in the fermentatio...
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An alkaliphilic microorganism from the genus Exiguobacterium, Exiguobacterium sp. strain HUD was isolated from a fermentative, methanogenic polymicrobial microcosm operating at pH 10. The draft genome shows the presence of genes encoding for the metabolism of a range of carbohydrates under both aerobic and anaerobic conditions.
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An alkaliphilic Bacillus isolate (strain JAMB-750) was found to exoproduce a novel alkaline mannanase (AmA). AmA was purified to homogeneity (about 98% pure) as judged by SDS-polyacrylamide electrophoresis. The molecular mass was approximately 130 kDa. The hydrolysis patterns of various substrates indicated that the enzyme was an endo-type enzyme. The optimal temperature and pH for activity wer...
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An alkaline mannanase (EC 3.2.1.78) from the alkaliphilic Bacillus sp. strain JAMB-602 was cloned and sequenced. The deduced amino-acid sequence of the enzyme suggested that the enzyme consists of a catalytic and unknown additional domains. The recombinant enzyme expressed by B. subtilis was crystallized using the hanging-drop vapour-diffusion method at 277 K. X-ray diffraction data were collec...
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